Macroporous bead modification with polyethylenimines of different molecular weights as polycationic ligands

摘要: Polyethylenimines (PEIs) with different molecular weights [number-average (Mn′s) = 60,000, 1200, and 423] were coupled onto macroporous beads. These rigid spherical beads prepared by the crosslinking of 2-hydroxyethyl methacrylate ethylene glycol dimethacrylate. The PEI attachment was carried out through epoxy groups yielded in a previous activation step epichlorohydrin on matrix hydroxyl groups. Different initial concentrations assayed. supports so obtained characterized several techniques (Fourier transform infrared spectroscopy, scanning electron microscopy, thermogravimetric analysis, mercury intrusion porosimetry). All PEI-containing used to analyze influence that weight, shape polycationic ligand (PEI), degree coupling matrices may have had efficiency retention bovine serum albumin protein as model biomolecule. In these assays, PEI-modified Mn 60,000 showed better results than those modified PEIs Mn's 1200 423. presence sparse long chains matrix, reason their highest accessibility toward large protein, resulted disposition functional groups, whereas more short other (Mn's 423) ligands would not have. © 2010 Wiley Periodicals, Inc. J Appl Polym Sci,