A novel homogeneous assay format utilising proximity dependent fluorescence energy transfer between particulate labels

摘要: Abstract Particle labels feature considerably higher specific activity than small molecule due to high number of label molecules incorporated in one unit. We developed a novel homogeneous assay relying on time-resolved detection fluorescence energy transfer between particulate labels. The long-lifetime fluorescent Eu(III)-chelate-doped donor nanoparticles and the prompt near-infrared TransFluoSphere™ acceptor particles possess activity, resulting great sensitivity potential. proximity required was achieved via antibody–antigen interactions, where model analyte prostate antigen (PSA), were coated with monoclonal anti-PSA-antibodies for discrete PSA epitopes. Due long-lived emission acceptors, sensitized detectable delayed measurement window free autofluorescence. these properties low non-specific binding particles, limit below 0.1 ng/mL variations, typically 0.1–7%, achieved. setup is applicable sensitive indirect competitive or non-competitive high-throughput screening.