Sensitive quantitative protein concentration method using luminescent resonance energy transfer on a layer-by-layer europium(III) chelate particle sensor.
作者： Harri Härmä , Lars Dähne , Sari Pihlasalo , Janne Suojanen , Jouko Peltonen
DOI: 10.1021/AC801960C
关键词: Europium 、 Adsorption 、 Particle 、 Förster resonance energy transfer 、 Luminescence 、 Acceptor 、 Chemistry 、 Resonance (particle physics) 、 Analytical chemistry 、 Fluorescence spectrometry
摘要: A particle-based protein quantification method was developed. The relies on adsorption of proteins particles and time-resolved fluorescence resonance energy transfer (TR-FRET). Layer-by-layer (LbL) containing europium(III) chelate donor were prepared. labeled with an acceptor adsorbed onto the near-infrared signal detected in time-gated detection mode. Sample efficiently occupied particle surface preventing binding acceptor-labeled leading to a sensor significant change. We subnanomolar concentration using rapid simple mix-and-measure coefficient variation below 10%. Compared known methods, developed required no hazardous substances or elevated temperature reach high-sensitivity level.
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