Sensitive Luminometric Method for Protein Quantification in Bacterial Cell Lysate Based on Particle Adsorption and Dissociation of Chelated Europium

摘要: A sensitive and rapid assay for the quantification of proteins, based on sample protein adsorption to Eu3+-chelate-labeled nanoparticles, was developed. The lanthanide ion surface-conjugated Eu3+ chelate is dissociated at a low pH, decreasing luminescence signal. increased concentration prevents dissociation chelate, leading high signal due nanoparticle-bound protein. sensitivity proteins 130 pg bovine serum albumin (BSA), which an improvement nearly 100-fold from most commercial methods. average coefficient variation BSA 8%. protein-to-protein variability sufficiently low; values varied within 28% nine different proteins. developed method relatively insensitive presence contaminants, such as nonionic detergents commonly found in biological samples. existing methods tested ...