Characterization of the Mechanisms of Transcription Termination by the Helicase Sen1
作者： Odil Porrua , Domenico Libri
DOI: 10.1007/978-1-4939-2214-7_19
关键词:
摘要: In vitro transcription systems have been widely used to study all the steps of from initiation termination and many transcription-coupled processes. Here we describe an in transcription-termination assay that for analysis mechanism by yeast helicase Sen1. this system, use highly purified proteins assemble ternary elongation complexes (RNA polymerase, DNA template, nascent RNA) on biotinylated is subsequently immobilized streptavidin beads. After allowing addition nucleotides, events can be detected quantified comparing amounts polymerases transcripts released templates reactions performed absence or presence By modifying different parameters assay, technique allows several aspects reaction.
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Lidia Vasiljeva, Stephen Buratowski, Nrd1 interacts with the nuclear exosome for 3' processing of RNA polymerase II transcripts. Molecular Cell. ,vol. 21, pp. 239- 248 ,(2006) , 10.1016/J.MOLCEL.2005.11.028
F. William Studier, Protein production by auto-induction in high-density shaking cultures Protein Expression and Purification. ,vol. 41, pp. 207- 234 ,(2005) , 10.1016/J.PEP.2005.01.016
Marilyne Thiebaut, Elena Kisseleva-Romanova, Mathieu Rougemaille, Jocelyne Boulay, Domenico Libri, Transcription Termination and Nuclear Degradation of Cryptic Unstable Transcripts: A Role for the Nrd1-Nab3 Pathway in Genome Surveillance Molecular Cell. ,vol. 23, pp. 853- 864 ,(2006) , 10.1016/J.MOLCEL.2006.07.029
Odil Porrua, Fruzsina Hobor, Jocelyne Boulay, Karel Kubicek, Yves D‧Aubenton-Carafa, Rajani Kanth Gudipati, Richard Stefl, Domenico Libri, In vivo SELEX reveals novel sequence and structural determinants of Nrd1-Nab3-Sen1-dependent transcription termination. The EMBO Journal. ,vol. 31, pp. 3935- 3948 ,(2012) , 10.1038/EMBOJ.2012.237
Jonathan S. Finkel, Karen Chinchilla, Doris Ursic, Michael R. Culbertson, Sen1p Performs Two Genetically Separable Functions in Transcription and Processing of U5 Small Nuclear RNA in Saccharomyces cerevisiae Genetics. ,vol. 184, pp. 107- 118 ,(2010) , 10.1534/GENETICS.109.110031
Karen Chinchilla, Juan B. Rodriguez-Molina, Doris Ursic, Jonathan S. Finkel, Aseem Z. Ansari, Michael R. Culbertson, Interactions of Sen1, Nrd1, and Nab3 with Multiple Phosphorylated Forms of the Rpb1 C-Terminal Domain in Saccharomyces cerevisiae Eukaryotic Cell. ,vol. 11, pp. 417- 429 ,(2012) , 10.1128/EC.05320-11
J.-P. Hsin, J. L. Manley, The RNA polymerase II CTD coordinates transcription and RNA processing Genes & Development. ,vol. 26, pp. 2119- 2137 ,(2012) , 10.1101/GAD.200303.112
Maria L. Kireeva, Natalia Komissarova, David S. Waugh, Mikhail Kashlev, The 8-nucleotide-long RNA:DNA hybrid is a primary stability determinant of the RNA polymerase II elongation complex. Journal of Biological Chemistry. ,vol. 275, pp. 6530- 6536 ,(2000) , 10.1074/JBC.275.9.6530
Guillaume Rigaut, Anna Shevchenko, Berthold Rutz, Matthias Wilm, Matthias Mann, Bertrand Séraphin, None, A generic protein purification method for protein complex characterization and proteome exploration. Nature Biotechnology. ,vol. 17, pp. 1030- 1032 ,(1999) , 10.1038/13732
D J DeMarini, M Winey, D Ursic, F Webb, M R Culbertson, SEN1, a positive effector of tRNA-splicing endonuclease in Saccharomyces cerevisiae. Molecular and Cellular Biology. ,vol. 12, pp. 2154- 2164 ,(1992) , 10.1128/MCB.12.5.2154