Hydration-dependent conformational states of hemoglobin Equilibrium and kinetic behavior
作者： Marcio F. Colombo , Rosemary Sanches
DOI: 10.1016/0301-4622(90)85004-P
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摘要: Abstract The equilibrium and kinetics of methemoglobin conversion to hemichrome induced by dehydration were investigated visible absorption spectroscopy. Below about 0.20 g water per hemoglobin only was present in the sample; above this value, an increasing proportion appeared with increase hydration. transition between two derivatives showed a time-dependent biphasic behavior observed be reversible. rates obtained for 0.31 1.93 min −1 0.05 0.47 . We suggest that is reversible conformational state reflect α- β-chains hemoglobin.
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