Cross-linked bis-hemoglobins: connections and oxygen binding.

摘要: Covalently linked pairs of cross-linked hemoglobin tetramers (“bis-tetramers”, shown schematically as 6−8) were prepared by reacting A with tetrakis acyl phosphate esters (3−5). The effects the link between are observed in oxygen-binding properties bis-tetramers:  they bind oxygen cooperatively but Hill coefficients (n50) lower than that native protein and a high average affinity. bis-tetramers longer connections show higher n50, suggesting steric interactions affect cooperativity. These results correlate to reduced vasoactivity heterogeneous solutions oligomeric tetramers.