eDNA-based crayfish plague detection as practical tool for biomonitoring and risk assessment of A. astaci -positive crayfish populations

摘要: While environmental DNA (eDNA) approaches have the potential to revolutionize biodiversity research and monitoring, most aquatic biomonitoring management programs still rely on conventional monitoring methodologies. Here we evaluated suitability robustness of an eDNA-based approach for detection crayfish plague agent Aphanomyces astaci assessed capacity this new method as practical tool bioassessments in freshwater. The was examined three case studies: study A tested its use determine range density A. astaci- infected populations different water systems. Case B focussed identification possible infection sources, here aquafarm. For C a migration front near native area. allowed infer local patterns distribution limits occurrence. Spore estimates eDNA samples correlated significantly with catch per unit effort values pathogen loads captured positive obtained from trapping running waters. We also showed that spore concentrations are detectable up kilometres downstream hot spot areas crayfish. By identifying entry point proved suitable spores at low population densities and/or levels provides conclusive evidence risk assessment large-scale wide conservation issues indigenous species.