Methods for performing chemical assays using fluorescence and photon counting

摘要: Improved methods for determining very low concentrations of substances present in fluid samples are provided by employing light emitting tracer compounds and (1) counting the photons emitted therefrom while discriminating against noise, nonspecific light, quenching effects sample, or (2) over a predetermined integrated flux, combination (2). Further, novel fluorescently labeled molecular weight antigens which can be employed competitive binding techniques above described photon useful. A homogeneous assay, materials, eliminates need separating bound from unbound materials is also provided. Finally, modified enzyme amplification technique set forth enzymes active phase to provide assay useful extremely concentration assays.