Stem Cell Banks: Preserving Cell Lines, Maintaining Genetic Integrity, and Advancing Research

摘要: The ability to cryopreserve and successfully recover cell lines has been critical the conservation of all lines, especially preservation pristine early-stage cultures preparation well-characterized banks. Indeed, systematic storage establishment cryopreserved banks cells for stem research community is fundamental promotion standardisation in their use clinical applications. In spite significant potential therapy, they are challenging maintain have shown be unstable after prolonged culture that often results permanent alterations genetic make-up, which ultimately alters phenotype culture. This chapter will review principles bank production, techniques scale-up human pluripotent cells, quality control, characterisation methods banked lines.