Mouse natural killer subsets defined by their target specificity and their ability to be separately rendered unresponsive in vivo.

摘要: NK cells from F1(AxB) hybrid mice are known to reject bone marrow grafts either parent A or B (hybrid resistance). Using cold target competition in an vitro resistance assay, we demonstrate this work that and killed by different subsets. As confirmation of the existence these subsets, determine whether they undergo a selection/education process during cell ontogeny, constructed chimeras which NK1.1-depleted F1 were allowed mature microenvironment B. These F1-reconstituted shown have normal numbers lytic ability terms YAC-1 killing Ab-mediated redirected lysis. three-color flow cytometry analysis vivo found targets, but not less effectively removed develop recipient (F1-->A chimeras) than F1-->F1 chimeric mice. IL-2-activated killer cultures chimeras, however, did show significant difference anti-parent response assay. results suggest exist subsets self-reactive can be rendered unresponsive radioresistant host element(s). However, unresponsiveness broken when environment.