Cloning, expression of the human substance K receptor, and analysis of its role in mitogenesis.
作者： CURT CYR , VICTORIA SOUTH , ALAN SALTZMAN , S. FELDER , GEORGE A. RICCA
DOI: 10.1111/J.1749-6632.1991.TB33144.X
关键词:
摘要: The primary strudure of the human substance K receptor was established from sequences complementary DNA clones isolated a jejunal library. It consists 398 amino acids, including seven putative transmembrane regions. gene for localized to chromosome region 10p13-10q23, with frequent chromosomal abnormalities. expressed in transfeded NIH-3T3 cells lacking endogenous receptors, and Scatchard analysis 125I labeled binding indicates approximately 100,000 receptors/cell single dissociation constant 12 nM. Covalent cross-linking experiments utilizing 125I-substanceK three different chemical reagents (disuccinimidyl suberate, disuccinimidyl tartrate, or 1-ethyl-3-(3dimethylaminopropyl)carbodiimide-HCI) demonstrate an apparent molecular weight 45,000, consistent little no N-linked glycosylation. its on led rapid increase produdion total inositol phosphates release Ca2� internal stores. Growth is stimulated by addition medium level similar 10% serum. Therefore, can function as growth fador when mouse 3T3 cells.
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