Stress-Induced Nuclear RNA Degradation Pathways Regulate Yeast Bromodomain Factor 2 to Promote Cell Survival

摘要: Bromodomain proteins are key regulators of gene expression. How the levels these factors regulated in specific environmental conditions is unknown. Previous work has established that expression yeast factor 2 (BDF2) limited by spliceosome-mediated decay (SMD). Here we show BDF2 subject to an additional layer post-transcriptional control through RNase III-mediated (RMD). We found III Rnt1p cleaves a stem-loop structure within mRNA down-regulate its However, two nuclear RNA degradation pathways play distinct roles regulation expression, as RMD and SMD differentially activated or repressed conditions. hyper-activated salt stress hydroxyurea-induced DNA damage while inactivated predominates during damage. Mutations cis-acting signals rescue numerous growth defects cells lacking Bdf1p, plays important role response. These results demonstrate modulate Bdf2p-mediated regulation. Moreover, precise dosage essential for cell survival conditions, emphasizing their importance controlling chromatin response stress.