Single sperm cryopreservation on cryoloops: an alternative to hamster zona for freezing individual spermatozoa

摘要: Abstract Methodology enabling cryopreservation of individual spermatozoa in extreme cases oligozoospermia would tremendously benefit patients. This study explores the use a nylon loop for small quantities spermatozoa, and also describes novel technique freezing with this cryoloop. Experiments were conducted to compare sperm recovery viability after conventional vials versus on cryoloops. Discarded human specimens varying parameters utilized. The examines two different glycerol-based cryoprotectants, without test yolk buffer. For single cryopreservation, 5–10 selected loaded onto cryoloops aid microscope micromanipulation equipment. Sperm function testing was performed both bovine frozen Microquantities exhibited overall motility similar control samples cryovials. Individually cryopreserved loops easily recovered post-thaw generally good. demonstrated that able undergo head decondensation when injected into oocytes. Cryoloops may be an excellent alternative hamster zonae cryopreserving numbers spermatozoa.