Association between freezing agent and acrosome damage of human spermatozoa from subnormal and normal semen
作者： M. E. Hammadeh , T. Georg , P. Rosenbaum , W. Schmidt
DOI: 10.1046/J.1439-0272.2001.00462.X
关键词:
摘要: This experimental study compares the effects of human sperm preservation medium (HSPM) with TEST-yolk buffer (TYB) as cryoprotectants spermatozoa respect to integrity acrosome after freeze-thawing procedure. Fifty-six semen samples were included in this study; 18 subnormal (G1) and 38 normal (G2) based on World Health Organization criteria, except for morphology, which was evaluated according strict criteria. Each sample divided into two parts: first part prepared cryopreservation by addition HSPM (1:1) second TYB (1:1). Freezing performed liquid nitrogen vapour. Smears made before freezing thawing process evaluation using fluorescent-lectin labelling. The mean percentage intact acrosomes group 77.0 +/- 7.2% decreased significantly (P < 0.001) thawing: 63.7 8.2% use 66.8 8.7% TYB. corresponding values 83.4 9.2%, 76.0 8.8% 77.9 respectively. It is obvious that decrease higher when comparison TYB, not only G1 (-14.9 1.9% versus -11.8 1.4%) but also G2 (-13.8 1.5% -11.9 1.3%). In conclusion, should be recommended first-choice cryoprotectant, well samples, order protect from deleterious
sci-hub.se 参考文章(35)
Joho K. Critser, Angela R. Huse-Benda, David V. Aaker, Brian W. Arneson, G. David Ball, Cryopreservation of human spermatozoa. III. The effect of cryoprotectants on motility. Fertility and Sterility. ,vol. 50, pp. 314- 320 ,(1988) , 10.1016/S0015-0282(16)60079-1
Togas Tulandi, Robert A. McInnes, Vaginal lubricants: effect of glycerin and egg white on sperm motility and progression in vitro Fertility and Sterility. ,vol. 41, pp. 151- 153 ,(1984) , 10.1016/S0015-0282(16)47558-8
Gail S. Prins, Louise Weidel, A comparative study of buffer systems as cryoprotectants for human spermatozoa Fertility and Sterility. ,vol. 46, pp. 147- 149 ,(1986) , 10.1016/S0015-0282(16)49476-8
Roy H. Hammerstedt, John P. Nolan, James K. Graham, Cryopreservation of mammalian sperm: what we ask them to survive. Journal of Andrology. ,vol. 11, pp. 73- 88 ,(1990) , 10.1002/J.1939-4640.1990.TB01583.X
Yvon. Englert, Annick. Delvigne, Marcel. Vekemans, Bernard. Lejeune, Alain. Henlisz, Gisele. de Maertelaer, Fernand. Leroy, Is fresh or frozen semen to be used in in vitro fertilization with donor sperm Fertility and Sterility. ,vol. 51, pp. 661- 664 ,(1989) , 10.1016/S0015-0282(16)60617-9
Bayard T. Storey, Juan G. Alvarez, Evidence for increased lipid peroxidative damage and loss of superoxide dismutase activity as a mode of sublethal cryodamage to human sperm during cryopreservation. Journal of Andrology. ,vol. 13, pp. 232- 241 ,(1992) , 10.1002/J.1939-4640.1992.TB00306.X
LOUISE WEIDEL, GAIL S. PRINS, Cryosurvival of human spermatozoa frozen in eight different buffer systems Journal of Andrology. ,vol. 8, pp. 41- 47 ,(1987) , 10.1002/J.1939-4640.1987.TB02417.X
S. Y. W. CHAN, S. Q. LI, C. WANG, TEST-egg yolk buffer storage increases the capacity of human sperm to penetrate hamster eggs in vitro International Journal of Andrology. ,vol. 10, pp. 517- 524 ,(1987) , 10.1111/J.1365-2605.1987.TB00351.X
W. V. Holt, R. D. North, The role of membrane-active lipids in the protection of ram spermatozoa during cooling and storage. Gamete Research. ,vol. 19, pp. 77- 89 ,(1988) , 10.1002/MRD.1120190108
John E. Parks, T. N. Meacham, R. G. Saacke, Cholesterol and Phospholipids of Bovine Spermatozoa. I. Selection of a PIPES-Buffered Diluent for Evaluating the Effect of Egg Yolk Lipoproteins on Sperm Cholesterol and Phospholipids Biology of Reproduction. ,vol. 24, pp. 393- 398 ,(1981) , 10.1095/BIOLREPROD24.2.393