The inhibitory effects of cisplatin‑radiation combination treatment on malignant osteosarcoma MG‑63 cells and BRCA1‑p53 pathways are more efficient than single treatments

摘要: The poor prognosis of patients with osteosarcoma remains a persistent problem, in particular for unresectable tumors or metastasis. Therefore, combination radiotherapy and chemotherapy has been considered metastasis recurrence, unsuitable surgery refusing surgery. present study aimed to investigate the effect combined treatment cisplatin radiation therapy on biological characteristics cell line MG-63 breast cancer 1 (BRCA1)-associated signaling pathways. Cell proliferation was determined using Counting kit-8 assay, apoptosis cycle were assessed by flow cytometry. migration examined Transwell assay. mRNA protein expression levels candidate genes, including BRCA1 p53, reverse transcription-quantitative PCR western blotting, respectively. results demonstrated that significantly inhibited compared alone. Furthermore, radiation, increased rate cells, which resulted G2 phase arrest, decreased migratory capacity cells. In addition, cells following higher group, but lower group. p53. Additionally, had more potent inhibitory p53 than expression. Bax level inductive Bcl-2 treatments exhibited superior therapeutic effects alone, may be mediated BRCA1-p53 pathway.