作者: Rong Nie , Qing Zhou , Eman Jassim , Philippa T.K. Saunders , Rex A. Hess
DOI: 10.1095/BIOLREPROD66.4.1161
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摘要: Expression of estrogen receptors (ERs) in the reproductive tracts adult male dogs and cats has not been reported. In present study, ERa ERb were localized by immunohistochemistry using ER-specific antibodies. was found interstitial cells peritubular myoid dog testis, but only cat. rete testis dog, epithelial positive for staining, cat, epithelium weakly positive. efferent ductules both ciliated nonciliated stained intensely less than cells. Epithelial epididymis vas deferens negative ERa. except initial region caput epididymis, staining deferens. Multiple cell types cat testes ERb. ductules, Most exhibited a strong species. addition, double used to demonstrate colocalization The specificity antibodies demonstrated Western blot analysis. This study reveals differential localization tracts, demonstrating more intensive expression However, as other species, remained highest concentration estradiol receptor, tract,