Chapter 4 Manipulation of the Avian Segmental Plate in Vivo

摘要: Publisher Summary This chapter discusses the manipulation of avian segmental plate in vivo . The embryo provides a useful experimental system for investigation vertebrate development. To track fate experimentally manipulated cells embryo, cell-labeling techniques are required. When combined with tools molecular biology, lineage tracing is powerful tool analyzing mechanisms cell specification and morphogenesis. quail–chick grafting method developed by Le Douarin has been successfully used as technique embryo. Organ anlagen chick marked surgical replacement corresponding anlage from quail generated grafted can be identified Feulgen-stained tissue sections presence bright crimson nucleoli, which absent pale-staining nuclei host cells. presented this describes transplantation fragments plate, precursor to somites, embryonic skeletal muscle axial skeleton.