Molecular characterization revealed Fasciola specimens in Ecuador are all Fasciola hepatica, none at all of Fasciola gigantica or parthenogenic Fasciola species.
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DOI: 10.1016/J.PARINT.2020.102215
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摘要: Abstract All 225 Fasciola flukes obtained from domestic animals (73 cattle, 7 sheep and 1 pig) of 18 distinct geographic areas in Ecuador-South America, were identified as hepatica, based on molecular analyses nuclear pepck pold genes, mitochondrial nad1gene well the morphological observation sperm within seminal vesicles. gigantica parthenogenic forms endemic to Asian countries not found this study, although zebu cattle water buffalos have introduced into South America Asia; could be due absence suitable intermediate host snails. The results analysis using multiplex PCR developed previously showed that 32 confirmed F. suggesting method is unreliable for accurate discrimination gene species consists multiple loci, a single locus. genetic diversity, phylogenetic, network nad1 sequences suggest hepatica populations including Ecuador, formed ancestral individuals continent along with anthropogenic movement livestock infected species.
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