Telomere terminal transferase activity in the hypotrichous ciliate Oxytricha nova and a model for replication of the ends of linear DNA molecules

摘要: We have found abundant telomere-specific terminal transferase activity in crude macronuclear extracts from vegetatively growing cells of the hypotrichous ciliate Oxytricha nova. This adds two to seven tandem repeats sequence GGGGTTTT (the telomeric repeat) 3' end oligonucleotide primers ending G4T4 and always proper phase. The requires presence micromolar amounts dGTP dTTP as well single-stranded oligomer with sequence. A nuclease is present which closely balanced telomere activity. propose a simple model for replication ends linear DNA molecules based on transferase.