MALDI-TOF MS as a Novel Tool for Dereplication and Characterization of Microbiota in Bacterial Diversity Studies

摘要: Determining the number and identity of species strains present in a biological sample is main challenge microbial diversity studies. Traditionally, this involves first dereplication step which all isolates are examined to group those that represent same taxon rapid cost-effective manner. Dereplication therefore assessment novelty aims reduce large smaller, nonredundant set for further, more labor-intensive identification. The high-throughput capacity low running cost make matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF MS) most suited dereplication. Especially medical microbiology, availability commercial databases facilitates direct identification during increasingly precludes need further Commercial will have be expanded with appropriate numbers reference environmental food microbes order facilitate their studies as well. Alternatively, purpose-built constructed. communities strain level requires study but may become two-step procedure. Isolates grown on different media incubation conditions can identified at regardless these varying cultivation conditions. Yet, latter induce experimental variation spectra simultaneous use typing. In achieve latter, should recultivated standardized evaluate value individual peaks strain-specific markers.