Monoclonal antibodies to murine IL-1 alpha. Production, characterization, and inhibition of membrane-associated IL-1 activity.

摘要: We report the production of hamster anti-murine IL-1 alpha mAb and analysis their specificity suitability for use in murine immunologic biologic assays. The bound to (Mu) rIL-1 3 but not rMuIL-1 beta as assessed by both direct ELISA immunoprecipitation. They also inhibited activity MuIL-1 measured a T cell co-stimulator assay. These specific only partially found macrophage culture supernatants completely fixed peritoneal exudate cells. data indicate that species associated with membranes shares at least two epitopes probably represents product gene. reagents will be valuable quantitative assessment proteins on surfaces, supernatants, lysates. useful vitro vivo determining relative roles different development responses.