Autophagy is involved in oral rAAV/Aβ vaccine-induced Aβ clearance in APP/PS1 transgenic mice.

摘要: The imbalance between s-amyloid (As) generation and clearance plays a fundamental role in the pathogenesis of Alzheimer's disease (AD). sporadic form AD is characterized by an overall impairment As clearance. Immunotherapy targeting believed to be promising approach under active clinical investigation. Autophagy conserved pathway for degrading abnormal protein aggregates crucial We previously reported that oral vaccination with recombinant AAV/As vaccine increased from brain improved cognitive ability animal models, while underlying mechanisms were not well understood. In this study, we first demonstrated rAAV/As decreased p62 level up-regulated LC3B-II/LC3B-I ratio APP/PS1 mouse brain, suggesting enhanced autophagy. Further, inhibition Akt/mTOR may account autophagy enhancement. also found anti-As antibodies sera mice vaccination, accompanied elevation complement factors C1q C3 levels brain. Our results indicate closely involved vaccination-induced clearance, modulating important strategy prevention intervention.