Monoclonal antibodies distinguish between storage and secreted forms of eosinophil cationic protein

摘要: The toxic effects of eosinophils on parasites and cells are due largely to the secretion various granule proteins, following stimulation. In order study this secretory process (degranulation) further, we have raised mouse monoclonal antibodies against both human eosinophil extracts products. From immunocytochemical studies it appears that one antibody, EG1 , recognized storage secreted forms eosinophilcationic protein (ECP), whereas antibody EG2 only bound ECP during (and extraction). This also protein-X (EP-X). As stained in formalin-fixed tissues, they were used demonstrate sites activation chronic urticaria. capacity detect differences between proteins is an important property these reagents with many potential applications cell biology.