Simultaneous determination of unbound thyroid hormones in human plasma using high performance frontal analysis with electrochemical detection
作者： Tomoko Kimura , Keiko Nakanishi , Terumichi Nakagawa , Akimasa Shibukawa , Katsumi Matsuzaki
DOI: 10.1016/J.JPBA.2004.12.018
关键词:
摘要: A direct injection HPLC method in combination with high-performance frontal analysis (HPFA) and electrochemical detection (ECD) was developed for the simultaneous sensitive determination of unbound thyroid hormones (thyroxine, triiodothyronine, reverse triiodothyronine) human plasma. The present on-line HPLC/HPFA system consists an HPFA column, extraction column analytical connected through a column-switching device, eluent from monitored by ECD. calibration lines showed good linearity (rsq.>0.999) within 7.4-148.2 pM T4 1.5-74.1 T3 rT3. Unbound concentrations determined were 16.4+/-2.4 (n=15) 7.14+/-1.04 (n=15), which agreement those EIA method. rT3 concentration 2.30+/-0.27 (n=15). CV% values intra-day inter-day assays less than 14.9% T4, 14.5% 13.2% also applied to competitive binding study these
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