A KINETIC MODEL FOR THE INFLUENCE OF SERUM IN BATCH AND CONTINUOUS HYBRIDOMA CULTURES
作者： J.L. GOERGEN , A. MARTIAL , A. MARC , J.M. ENGASSER
DOI: 10.1016/B978-0-7506-1103-9.50118-9
关键词:
摘要: ABSTRACT The kinetics of batch and continuous cultures hybridoma has been investigated at levels foetal calf serum varying from 10 to 05 % v/v. A model taking into account the limiting effect on cell growth increased glucose uptake reduced correctly describes metabolism in two culture modes . KEY WORDS : Hybridoma, serum, kinetics, modelling, cultures,
sci-hub.st 参考文章(3)
S. Ismet G�rhan, Nilg�n �zdural, Serial cultivation of suspended BHK 21/13 cells in serum-reduced and serum-free medium supplemented with various membrane protective agents. Cytotechnology. ,vol. 3, pp. 89- 93 ,(1990) , 10.1007/BF00365269
John P. Tharakan, Alexander Lucas, Pao C. Chau, Hybridoma growth and antibody secretion in serum-supplemented and low protein serum-free media. Journal of Immunological Methods. ,vol. 94, pp. 225- 235 ,(1986) , 10.1016/0022-1759(86)90237-1
Mina Dalili, David F. Ollis, Transient kinetics of hybridoma growth and monoclonal antibody production in serum-limited cultures. Biotechnology and Bioengineering. ,vol. 33, pp. 984- 990 ,(1989) , 10.1002/BIT.260330807