Advances in protoplast research on Solanum
作者： David I. Ferreira , Aaron Zelcer
DOI: 10.1016/S0074-7696(08)60627-0
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摘要: Publisher Summary This chapter describes the developments in protoplast research genus Solanum . protoplasts are isolated from tubers, callus cultures, and cell suspension mainly leaf mesophyll tissue. The physiological state of plant material is important for reliable isolation, culture, regeneration. source can contribute to extent protoclonal variation. aim regeneration achieved by using various sources grown under different conditions. Protoclonal variation seems occur more cultures derived glasshouse-grown than shoot cultures. isolation procedure depends on type used. treated ways, prior incubation an enzyme solution. employed phytopathological fungal virus diseases, potential lies use improvement. Plants regenerated a large number genotypes way utilize these protoclones breeding attempted. Regenerating plants species clones provide tool better understanding differentiation
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