Antidiabetic activity of 3-hydroxyflavone analogues in high fructose fed insulin resistant rats.

摘要: Synthetic 3-hydroxyflavone analogues (JY-1, JY-2, JY-3, JY-4), were tested for antidiabetic activity in high-fructose-diet-fed (66 %, 6 weeks) insulin-resistant Wistar rats (FD-fed rats). The fasting blood glucose, insulin, creatinine and AGEs decreased to near normal upon treatment with test compounds. Insulin resistance markers such as HOMA-IR, K-ITT, plasma triglycerides, lipids, endogenous antioxidant defense glycogen restored FD-fed after 3-hydroxyflavones. It is known that insulin partly because of oxidative stress hence was determined. They exhibited significant vitro DPPH ABTS radical scavenging (IC50: 10.66-66.63 µM). Test compounds inhibited ROS NO production RAW 264.7 cells 10.39-42.63 µM) they found potent quercetin. Further, the lipid peroxidation at low concentrations 99.61-217.47 All 100-200 µM protected calf thymus DNA-damage by Fenton reaction. In addition, protein glycation different antiglycation assays. JY-2 showed maximum potency all stages which comparable standard quercetin aminoguanidine. also enhanced glucose uptake L6 myotubes an EC50 much lower than Thus synthetic 3-hydroxyflavones have good pleotropic multimodal suppression enhancement skeletal muscles. These are non-toxic doses tested. combined activities these molecules complementary benefits management diabetes.