Strategies for the discovery and engineering of enzymes for biocatalysis.
作者： Timo Davids , Marlen Schmidt , Dominique Böttcher , Uwe T Bornscheuer
DOI: 10.1016/J.CBPA.2013.02.022
关键词:
摘要: Protein engineering is the most important method to overcome limitations of natural enzymes as biocatalysts. The past few years have seen a tremendous increase in novel concepts facilitate design mutant libraries for focused directed evolution mostly guided by advanced bioinformatic tools. In addition, high-throughput methods were developed using, example, FACS analysis or microfluidic systems. These achievements significantly tailor-made make them suitable industrial applications.
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