Characterization of the Rabbit Neonatal Fc Receptor (FcRn) and Analyzing the Immunophenotype of the Transgenic Rabbits That Overexpresses FcRn

摘要: The neonatal Fc receptor (FcRn) regulates IgG and albumin homeostasis, mediates maternal transport, takes an active role in phagocytosis, delivers antigen for presentation. We have previously shown that overexpression of FcRn transgenic mice significantly improves the humoral immune response. Because rabbits are important source polyclonal monoclonal antibodies, adaptation our technology this species would bring significant advantages. cloned full length cDNA rabbit alpha-chain found it is similar to its orthologous analyzed so far. - contact residues highly conserved, based on we predicted pH dependent interaction, which confirmed by analyzing binding using yolk sac lysates fetuses Western blot. Using immunohistochemistry, detected strong staining endodermal cells membrane, while placental trophoblast amnion showed no staining. Then, BAC transgenesis generated carrying overexpressing a 110 kb genomic fragment encoding FcRn. These – having one extra copy when hemizygous two copies homozygous improved protection augmented response immunized with variety different antigens. Our results these demonstrate increased response, what described mice, indicating brings advantages production antibodies.