Regeneration of supraspinal axons after complete transection of the thoracic spinal cord in neonatal opossums (Monodelphis domestica).

摘要: These studies define the time table and origin of supraspinal axons regenerating across a complete spinal transection in postnatal Monodelphis domestica. After lumbar (L1) cord injection fluorophore–dextran amine conjugate on (P) day 4, consistent number neurons could be labeled. The numbers labeled remained stable for several weeks, but subsequently declined by P60 control animals P35 with (T4–T6) performed at P7. In animals, 25–40% fluorophore injected P4 also double-labeled second (T8–T10) different older ages. spinally transected total marker were initially lower compared age-matched controls, not significantly 3 weeks after injury. proportion increased from approximately 2% 1 week injury (P14) to 50% P60, indicating that substantial P7 is able regenerate persist into adulthood. However, originating made only small contribution ages fibers growing through site, because much development occurs Evidence was obtained degenerating both apoptotic necrotic morphologies present brainstem nuclei; morphology greater cords J. Comp. Neurol. 466:422–444, 2003. © 2003 Wiley-Liss, Inc.