Fungus culture and PCR in nasal lavage samples of patients with chronic rhinosinusitis

摘要: Chronic rhinosinusitis (CRS) affects approximately 15 % of the adult population in industrialized countries. Fungi have been recognized as important pathogens CRS immunocompromised host. Recently, fungi detected more than 90 nasal lavages (NLs) immunocompetent patients with CRS. Employing NLs present study, detection rates for by culture techniques were compared results different fungus-specific PCR assays. Standard fungal cultures performed on from 77 also tested presence DNA a panfungal assay and without specific probes Candida spp. Aspergillus spp./Penicillium spp., an Aspergillus-specific nested assay. Nineteen samples (25 %) grew fungi. Fungus-specific was 34 (44 %). Twelve positive both PCR, whereas seven specimens culture, but negative additional culture. The combination all employed assays 39 (50 This study demonstrated that conventional could be complementary diagnostic to detect patients.