Eight-plasmid rescue system for influenza A virus

摘要: Abstract Plasmid-driven synthesis of viral RNA and protein allows the recovery infectious influenza virus without need for helper infection. Because no selection system is required this approach, genetic manipulation all eight gene segments technical limitations possible. We have developed a which requires construction transfection only plasmids A viruses. In DNA system, cDNA inserted between human polymerase I (pol I) promoter murine terminator sequences. The entire pol transcription unit flanked by an II II) poly(A) site. As first step to evaluate utility plasmid-based production vaccines, we generated master strain A/PR/8/34 (H1N1) currently used inactivated vaccines entirely from cloned cDNAs. yield as determined HA-assay after passage recombinant in eggs was high parental wild-type virus. These results prove that has same growth properties egg grown indicate eight-plasmid method potential improve methods vaccine