Expansion of CD4+CD25+FoxP3+ regulatory T cells in chronic hepatitis C virus infection.
作者： Shahin Merat , Hediyeh Zamini , Leila Nemati , Hossain Jabbari , Ehsan Janzamin
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摘要: Background: Regulatory T cells (Tregs) have been involved in impaired immunity and may a pivotal role persistence of viral infections. Objective: To develop simple reliable in-house three color flow cytometery peripheral blood to understand the HCV infection increase Tregs. Methods: The level naturally occurring CD4+CD25+FoxP3+ regulatory (nTregs) 20 chronically infected with hepatitis C virus (HCV) patients was compared those 15 healthy individuals by flowcytometry. In different approach we performed permeabilization intracellular staining before surface which allows preservation molecules combined detection process results normal frequency nTregs blood. Results: Using optimized method, it shown that significantly higher proportion total CD4+ cell population seen chronic (0.83 ± 0.21%, p=0.05) as controls (0.26 0.1, p=0.05). Conclusions: accordance other studies, showed induces dramatic Tregs, might contribute immune response failure during infection.
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