Identification and characterization of a fatty acyl reductase from a Spodoptera littoralis female gland involved in pheromone biosynthesis.
作者： G. Carot-Sans , L. Muñoz , M. D. Piulachs , A. Guerrero , G. Rosell
DOI: 10.1111/IMB.12138
关键词:
摘要: Fatty acyl-CoA reductases (FARs), the enzymes that catalyse reduction of a fatty to corresponding alcohol in insect pheromone biosynthesis, are postulated play an important role determining proportion each component blend. For first time, we have isolated and characterized from Egyptian cotton leaf worm Spodoptera littoralis (Lepidoptera: Noctuidae) FAR cDNA (Slit-FAR1), which appeared be expressed only gland was undetectable other female tissues, such as fat body, ovaries, wings, legs or thorax. The encoded protein has been successfully recombinant system, enzyme is able produce intermediate acid alcohols biosynthesis S. littoralis precursors. kinetic variables Km Vmax, calculated for precursor, suggest biosynthetic (e.g. desaturases, acetyl transferase) should also contribute final ratio components In phylogenetic analysis, Slit-FAR1 grouped cluster FARs involved insects, with little non-specificity natural
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