Hydrogen production by the hyperthermophilic eubacterium, Thermotoga neapolitana, using cellulose pretreated by ionic liquid

摘要: Abstract The application of thermophilic bacteria to the production hydrogen (H 2 ) from a cellulosic biomass has captured attention engineering researchers. Thermotoga neapolitana is considered as potential producer because its ability be used for direct H fermentation raw cellulose, but yield not high. In this study, three chemical pretreatment methods with an ionic liquid, acid and alkali, respectively, were tested on cellulose enhance conversion strain into . Batch cultivations carried out investigate influence under non-sterile conditions. highest concentration in headspace obtained liquid-pretreated was 36.1% (v/v) ethanol anti-solvent. This clearly higher than that acid- alkali-pretreated celluloses (22–24%). Cultivation pretreated liquid at 10% combining N sparging showed maximum cumulative 1280 mL H /L culture. value approximately 10-fold (122 mL H culture). 2.2 mol H /mol glucose equivalents. results using considerable improving substrates by bacteria.