Evaluation of EVI1 and EVI1s (Δ324) as potential therapeutic targets in ovarian cancer

摘要: Abstract Purpose The MDS1 and EVI1 complex locus (MECOM) at 3q26 gives rise to several alternatively spliced transcripts implicated in leukemic oncogenesis. Overexpression of ovarian cancer has led a proposed oncogenic role. Our objective was evaluate the therapeutic potential EVI1s (also known as Δ324) cancer. Methods Expression mRNA protein isoforms evaluated cancers, normal ovaries, benign neoplasms, fallopian tube fimbria. Effects isoform overexpression knockdown on proliferation, cisplatin-induced apoptosis, double stranded DNA breaks were investigated. Results mRNAs ubiquitously expressed cancers gynecologic tissues examined, with highest expression both noted samples. total ratio uniform among examined tissues. In contrast, levels undetectable tissues, serous cancers. patterns similar between samples, fimbria, neoplasms. or did not increase proliferation EVI1-null OVCAR8 cells. Total selective expressing cells had no effect γ-H2AX Conclusion data do support role for cell response damage. Further research is required before can be considered an oncogene target