Quantitative comparison of the expression of EVI1 and its presumptive antagonist, MDS1/EVI1, in patients with myeloid leukemia.
作者： Ursula Vinatzer , Christine Mannhalter , Margit Mitterbauer , Helga Gruener , Hildegard Greinix
DOI: 10.1002/GCC.10144
关键词:
摘要: The EVI1 gene in chromosome band 3q26 exhibits a number of properties consistent with role as an oncogene, and its expression is activated most myeloid leukemia patients with, well minority without, rearrangements. A splice variant this gene, MDS1/EVI1, acts antagonist at least some tissue culture assays. We established real-time quantitative reverse transcriptase polymerase chain reaction (RTQ-RT-PCR) assays for these mRNA variants to compare their levels quantitatively reliable manner. was overexpressed highly variable extents all 14% In samples, MDS1/EVI1 also transcribed elevated compared those healthy controls. However, although the induction comparable to, or higher than, that three five samples normal locus, true only two 13 aberration. further provide preliminary evidence RTQ-RT-PCR assay may be useful disease monitoring overexpressing EVI1.
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