Changes in the levels of endogenous antioxidants in the liver of mice with experimental endotoxemia and the protective effects of the antioxidants.

摘要: Gram-negative bacterial endotoxin (lipopolysaccharide) induces hepatic damage in mice caused by lipid peroxidation, and administration of antioxidants (coenzyme Q10 alpha-tocopherol) suppresses this preserves energy metabolism, enhances the survival endotoxin-administered mice. Therefore experiments were done to determine whether experimental endotoxemia affected levels endogenous treatment with altered these levels. Endotoxin produced decreases time: reduced coenzyme Q9, alpha-tocopherol, glutathione lowered 29.9%, 27.1%, 45.4% control levels, respectively, 24 hours after administration. Exogenously administered (oxidized) accumulated liver showed a maximal plateau between 8 16 injection when 82% was converted form. Coenzyme totally or partially prevented antioxidants, furthermore, total form, both at approximately 30 mg protein, consumed during period elevated injection. These results indicate that acts vivo as an antioxidant it has been alpha-Tocopherol also 84-fold accumulation injection, completely preventing any decrease Q9 reduction glutathione, which indicated action alpha-tocopherol. support assumption alpha-tocopherol functions cooperatively prevent tissue peroxidation endotoxemia.