Crohn's disease monocytes are primed for accentuated release of toxic oxygen metabolites

摘要: Background: Inflammatory bowel disease occurs in regions of the intestine characterized by a content high bacteria. Intestinal bacteria synthesize cell wall products such as lipopolysaccharide; when normal monocytes or macrophages come contact with these products, they can be primed to release number inflammatory mediators. Mediators toxic oxygen metabolites released part respiratory burst may contribute tissue damage. The aim this study was determine if from patients Crohn's are lipopolysaccharide for greater burst. Methods: generation superoxide anion measured dismutase inhibitable reduction ferricytochrome c. Results: Freshly isolated active untreated (n = 8) showed enhanced stimulated compared 15; 3.80 ± 0.12 vs. 1.02 0.06 nmol/5 min; P < 0.001). We tested hypothesis that monocyte priming factor serum showing lost its ability prime after removed (0.25 0.25 min, Conclusions: These studies indicate bacterial important proinflammatory molecules involved initiation and/or perpetuation disease.