Relation Between Excreted Lipopolysaccharide Complexes and Surface Structures of a Lysine-Limited Culture of Escherichia coli

摘要: Knox, K. W. (Twyford Laboratories, London, England), Maret Vesk, and Elizabeth Work. Relation between excreted lipopolysaccharide complexes surface structures of a lysine-limited culture Escherichia coli. J. Bacteriol. 92:1206-1217. 1966.-The lysine-requiring mutant coli 12408, when grown in 15 liters defined medium containing suboptimal amount lysine, showed biphasic type growth. During long stationary phase hr, there was steady accumulation diaminopimelic acid (DAP) an antigenic complex (LPS) lipoprotein; the continued unchanged until end second growth phase. The rapid rate DAP excretion suggested that it result derepressed state biosynthetic pathway. LPS such fluid doubled during period corresponding to normal generation time for organism; this LPS-lipoprotein product unbalanced Surface defects were by action lysozyme, which, low concentrations (10 mug/ml), lysed cells even absence ethylenediaminetetraacetic acid, but had no effect at 10 mug/ml on with adequate lysine. Electron microscopy excreting them be surrounded mass stacked leaflets globules, some which bounded triple membranes. Sections lysis changes cell surfaces; outer layers walls numerous blebs whose membranes sometimes continuous membrane wall. probably originates from these blebs.