The primary neuronal cells are more resistant than PC12 cells to α-synuclein toxic aggregates

摘要: Abstract Background Alpha-synuclein (αSN) is an abundant presynaptic brain protein that its aggregated species believed to play pivotal roles in the development of neurodegenerative diseases, especially Parkinson’s disease (PD). In this study, we compared response primary neuronal cells with a well-known cell line model, PC12, against toxic aggregates αSN. Methods Primary hippocampal neurons (PHNs) were isolated from 17 18 days old rat embryos. Fibrillization was induced recombinant αSN and monitored by standard methods. The toxicity different on treated then studied. Furthermore, changes intracellular reactive oxygen (ROS) Ca2+ levels also two kinds cells. We studied gene expression profile certain channels carriers using GEO2 database. Results viability rate significantly lower PC12 versus PHNs, This while ROS increased both types. Analysis microarray data indicated some factors involved hemostasis may face significant PD condition. Conclusion By putting these together, it clear PHN more resistant than toward cytotoxicity even presence rising cytoplasmic levels. Exploring supporting mechanisms which uses be can help open roadmap therapeutic plans other synucleinopathy disorders.