Nuclear glutaredoxin 3 is critical for protection against oxidative stress-induced cell death.

摘要: Mammalian glutaredoxin 3 (Grx3) has been shown to be critical in maintaining redox homeostasis and regulating cell survival pathways cancer cells. However, the regulation of Grx3 is not fully understood. In present study, we investigate subcellular localization under normal growth oxidative stress conditions. Both fluorescence imaging Grx3–RFP fusion Western blot analysis cellular fractionation indicate that predominantly localized cytoplasm conditions, whereas oxidizing translocated into accumulated nucleus. nuclear accumulation was reversible a redox-dependent fashion. Further indicates neither N-terminal Trx-like domain nor two catalytic cysteine residues active CGFS motif are involved its translocation. Decreased levels render cells susceptible stress, overexpression nuclear-targeted sufficient suppress cells’ sensitivity oxidant treatments reduce reactive oxygen species production. These findings provide novel insights Grx3, which crucial for against environmental insults.