Blood-Testis Barrier Dynamics Are Regulated by α2-Macroglobulin via the c-Jun N-Terminal Protein Kinase Pathway

摘要: The blood-testis barrier (BTB), in contrast to the blood-brain and blood-retina barriers, is composed of coexisting tight junctions, gap basal ectoplasmic specializations, a testis-specific type adherens junction. Recent studies showed that BTB restructuring facilitates germ cell migration during spermatogenesis involves proteolysis, an event usually restricted cell-matrix interface other epithelia. For instance, surge alpha(2)-macroglobulin (alpha(2)-MG), protease inhibitor produced by Sertoli cells, was detected at Sertoli-Sertoli Sertoli-germ epithelium cadmium chloride-induced disruption adult rats. It thus proposed increase alpha(2)-MG crucial for protecting from unwanted proteolysis as well regulating availability cytokines affect junction turnover. Although both dynamics are regulated via p38 MAPK signaling pathway, mechanism(s) regulates entirely unknown. In this study, we have shown administering dimethylaminopurine, c-Jun N-terminal protein kinase (JNK) inhibitor, testis, JNK activity blocked specifically production inhibited, worsening damage epithelium. Studies coupled with inhibitors, immunoblottings, immunofluorescent electron microscopy unequivocally demonstrated pathway putative regulatory testis. This finding illustrates first time occurs normal physiology cell-cell highlighting significance regulation function.