Gonadotropin Releasing Hormone and Transforming Growth Factor β Activate Mitogen-Activated Protein Kinase/Extracellularly Regulated Kinase and Differentially Regulate Fibronectin, Type I Collagen, and Plasminogen Activator Inhibitor-1 Expression in Leiomyoma and Myometrial Smooth Muscle Cells

摘要: GnRH analog (GnRHa) and TGF-β act directly on leiomyoma/myometrial smooth muscle cells (LSMCs MSMCs) regulating diverse activities resulting in leiomyoma growth regression. Because receptor signaling is part mediated through the MAPK pathway, we determined whether contribution of MAPK/ERK transcriptional activation c-fos c-jun, result differential regulation type I collagen, fibronectin, plasminogen activator inhibitor 1 (PAI-1) gene expression, whose products are known to influence extracellular matrix turnover, which critical GnRHa-induced We found that GnRHa a dose- time-dependent manner increased level phosphorylated ERK1/2 (pERK1/2) LSMCs MSMCs. nuclear accumulation c-jun mRNA expression via downstream from kinase (MEK)1/2, because pretreatment with U0126, synthetic MEK1...