Gonadotropin releasing hormone analogue therapy alters signal transduction pathways involving mitogen-activated protein and focal adhesion kinases in leiomyoma

摘要: Because gonadotropin releasing hormone analogue (GnRHa) therapy often causes leiomyoma regression, in part through alteration of growth factor and receptor expression, we determined whether GnRHa alters the expression extracellular signal-regulated kinase (ERK) focal adhesion (FAK), which are linked to intracellular signaling pathways activated by ovarian steroids, factors, molecules. Leiomyoma matched unaffected myometrium were collected from women who received (n = 5) untreated 10). We ERK1, ERK2, FAK, phosphorylated ERK (pERK1/2), pFAK using Western blotting immunohis-tochemical analysis. expressed ERK1 (44 kD), ERK2 (42 FAK (125 kD) at variable levels with increased pERK, leiomyoma. found that resulted a noticeable decrease significant reduction pERKl/2 low or undetectable both compared group (P <.05). Immunohis chemically pERKl/2, localized smooth muscle cells connective tissue fibroblasts GnRHa-treated myometrium, considerable their intensity as indicated HScore tissues. The data provide further evidence regression induced is mediated mechanism involving suppression signal transduction factors steroid