Immunostaining for activated extracellular signal-regulated kinases in cells and tissue

摘要: Publisher Summary This chapter describes immunostaining in cells and tissues using a phospho- mitogen-activated protein (MAP) kinase specific antibody. These activation state-specific reagents enable one to assess the intracellular localization of active MAP thus understand ways signal transduction is regulated spatially temporally. The state antibodies can also reveal cellular heterogeneity within organs be informative about intercellular communication. ability assay signaling molecules paraffin-embedded human pathology specimens opens up possibility examining dynamics regulation disease development, providing powerful adjunct traditional tools pathologist, which primarily examine expression. Use these should increase our knowledge biochemistry proteins role play normal physiology processes.