Finite proliferative lifespan in vitro of a human breast cancer cell strain isolated from a metastatic lymph node

摘要: We recently described culture conditions that allow proliferation of metastatic human breast cancer cells from biopsy specimens certain patient samples. These resulted in the development an immortalized cell strain designated SUM-44PE. same were used to isolate a lymph node separate patient. The SUM-16LN isolated this specimen cultured either serum-free medium or serum-containing supplemented with insulin and hydrocortisone. Unlike SUM-44PE have proliferated continuously for over two years, approximately 200 days underwent 15 20 population doublings before undergoing senescence. No beyond passage 8. keratin-19 positive had aneuploid karyotype. overexpressed p53 protein amplified epidermal growth factor (EGF) receptor gene high level expression tyrosine phosphorylated EGF protein. Despite presence levels these cells, they serum-free, EGF-free did not secrete detectable EGF-like mitogenic factor. In addition, potently inhibited by all concentrations exogenous tested neutralizing antibody Mab 425. results suggest present is direct result overexpression simulatory ligand. Thus, represents exhibited genetic cellular characteristics advanced cells. Nevertheless, finite proliferative lifespan culture, suggesting immortalization phenotype expressed