Establishment and characterization of two primary breast cancer cell lines from young Indian breast cancer patients: mutation analysis

摘要: Two novel triple negative breast cancer cell lines, NIPBC-1 and NIPBC-2 were successfully established from primary tumors of two young patients aged 39 38 years respectively, diagnosed as infiltrating duct carcinoma breast. Characterization these lines showed luminal origin with expression epithelial specific antigen cytokeratin 18 presence microfilaments secretary vesicles, microvilli, tight junctions desmosomes on ultra-structural analysis. Both the anchorage independent growth invasion matrigel coated membranes. Karyotype analysis aneuploidy, deletions multiple rearrangements in chromosomes 7, 9, X 11 isochromosomes 17q both lines. P53 mutational revealed no mutation coding region lines; however line heterozygous C/G polymorphism, g.417 C > G (NM_000546.5) resulting Arg/Pro allele at codon 72 exon 4. Screening for mutations BRCA1&2 genes three polymorphisms BRCA1 2 exons 11, and14 BRCA2 gene CD 44+/24-breast stem cells capability producing mammosphere culture. The early onset can serve invitro models to study mechanisms underlying tumorigenesis younger age group also identification new therapeutic modalities targeting cells.