Establishment and characterization of three new breast-cancer cell lines

作者: Brigitte Siwek , Denis Larsimont , Marc Lacroix , Jean-Jacques Body

DOI: 10.1002/(SICI)1097-0215(19980529)76:5<677::AID-IJC11>3.0.CO;2-1

关键词: CytokeratinMolecular biologyAntigenEndoplasmic reticulumCell cultureBiologyVimentinReceptorMammary tumorEstrogen receptorPathology

摘要: We have established and characterized 3 new breast-cancer cell lines from pleural effusions of patients with advanced breast cancer. All lines, designated IBEP-I, IBEP-2 IBEP-3, showed typical ultrastructural characteristics epithelial mammary tumor cells. Electron microscopy showed, among other characteristics, the presence numerous microvilli, desmosomal junctions, intracytoplasmic duct-like vacuoles, well-developed endoplasmic reticulum large nuclei. Immunohistochemical biochemical studies revealed that expressed cytokeratin, membrane antigen, CEA CA 15-3, but all negative immunoreaction for vimentin. On hand, antigens (LEU-M1, GCDFP 15, c-erbB-2) were by some in a variable manner. Ploidy confirmed neoplastic origin lines. The doubling times 68 hr IBEP-1, 29 39 IBEP-3. Only cells estrogen receptors (ER + ), which down-regulated after pre-incubation E 2 , they did not express progesterone (PgR-). IBEP-1 IBEP-3 ER- PgR (PgR ). In these (10 -8 M) 24 hr. Estradiol (E ) increased proliferation rate -3 S.C. injection IBEP into nude mice resulted growth solid tumors between II 16 weeks inoculation. These could thus be models studying various aspects biology tumorigenicity A major interest is them ER - an exceptional phenotypic feature. interesting regulation effects progestins antiprogestins independently ER.

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